Journal: Membranes

Article Title: Finned Tubular Air Gap Membrane Distillation

doi: 10.3390/membranes13050498

Figure Lengend Snippet: Parameters of the test membrane and the finned tubular air gap membrane distillation module used in this work. ( a ) The micromorphology of the test PTFE membrane characterized by SEM. ( b ) Schematic diagram of the finned tubular air gap membrane distillation module. ( c ) Schematic diagrams of finned tubular air gap membrane distillation tests.

Article Snippet: Commercial polytetrafluoroethylene (PTFE) membranes (Membrane Solutions, LLC, Auburn, WA, USA) were used in finned tubular air gap membrane distillation tests.

Techniques: Membrane, Distillation

Journal: International Journal of Molecular Sciences

Article Title: A Novel Mouse Monoclonal Antibody C42 against C-Terminal Peptide of Alpha-1-Antitrypsin

doi: 10.3390/ijms22042141

Figure Lengend Snippet: A full-length AAT protein and C-36 peptide separated by 16.5% SDS-PAGE gels, followed by Western blotting using polyclonal rabbit anti-human AAT (anti-AAT) and mouse monoclonal anti-C-36 peptide antibody (C42). Protein samples prepared for SDS-PAGE analysis were denatured by heating at 90 °C for 3 min. After electrophoretic separation, proteins were transferred into 0.2 µm polyvinylidene difluoride (PVDF) membranes. Membranes were blocked for 1 h with TBS+ 0.01% Tween-20 containing 5% low fat milk powder and incubated overnight at 4 °C with rabbit polyclonal anti-human AAT (1:800) or C42 mAb (1:500). The immune complexes were visualized with appropriate horseradish peroxidase-conjugated secondary antibodies and enhanced by ECL Western blotting substrate. Images obtained with Chemidoc Touch imaging system. Representative blot shows that polyclonal anti-AAT antibody reacts with both -native AAT and C-36 peptide, whereas under the same experimental conditions, C42 mAb does not recognize proteins.

Article Snippet: The full-length AAT protein and C-36 peptide were heated at 90 °C for 3 min and separated by 16.5% SDS-PAGE (Bio-Rad, Hercules, CA, USA) prior to transfer onto 0.2 μm polyvinylidene difluoride (PVDF) membranes (SERVA, Heidelberg, Germany).

Techniques: SDS Page, Western Blot, Incubation, Imaging